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Formula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. AFORMULA REFERENCE GUIDEFOR SOFTMAX® PRO SOFTWAREMolecular Devices Corporation

1. IntroductionFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A6tion is processed from inside to outside, starting with the

Formula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A72. OperatorsChapter 2 describes the different types of operators that can be

2. OperatorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A82.1.1. USING MATHEMATICAL OPERATORS The following two examples

2.2. Comparison (Logical) OperatorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A9The formula for AdjResults can be written

2. OperatorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A10Example: Reporting Whether Data Reaches a Threshold ValueCreat

2.3. Conditional (Boolean) OperatorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A112.3. CONDITIONAL (BOOLEAN) OPERATORS Co

2. OperatorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A122.3.1. USING COMPARISON AND CONDITIONAL OPERATORSConditional f

2.3. Conditional (Boolean) OperatorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A13In this formula, if Condition#1 is true

2. OperatorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A14DiagramExample: A Conditional Formula with One Condition and T

2.3. Conditional (Boolean) OperatorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A15Example: A Conditional Statement with T

Formula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A2Molecular Devices CorporationFormula Reference Guide forSoftMax Pro Softwar

2. OperatorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A16Likewise, if a conditional statement evaluates to text, you ca

2.4. Concatenation OperatorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A17The difference between ~ and & is illustrat

2. OperatorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A18from a Spectrum scan. We can define a new column MaxOD to repo

Formula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A193. FunctionsThe general format for the many SoftMax Pro built-in functions

3. FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A20Trigonometric functions are calculated in radians (180 degrees

3.1. Mathematical FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A21Ceil(Parameter) Returns a number, list of numbe

3. FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A22Int(Parameter)Returns the integer part of a number, list of nu

3.1. Mathematical FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A23The functions above can be used singly as shown

3. FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A243.2. STATISTICAL FUNCTIONS Statistical functions behave differ

3.3. Interpolation FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A25For example, if you want the maximum values in

Formula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. AiContents1. IntroductionFormula Categories . . . . . . . . . . . . . . . . .

3. FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A26AreaUnderFit(PlotName, Xstart, Xstop)Returns the area under th

3.3. Interpolation FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A27ChiProbabilityPLA(PlotName@GraphSection)Returns

3. FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A28InterpY( PlotName@GraphName, Xvalues) Returns the Y values of

3.3. Interpolation FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A29Example: Using InterpX and InterpY in Group Sec

3. FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A303.4. VMAX REDUCTION FUNCTIONS You can determine VMax Rate or T

3.4. VMax Reduction FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A31VMax(Parameter1, Parameter2, Parameter3) Retur

3. FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A32Example: Viewing VMax Rate Determined Using Different Numbers

3.4. VMax Reduction FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A33Example: Viewing the VMax Correlation Coeffici

3. FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A343.5. OTHER FUNCTIONS The functions listed below allow you to a

3.5. Other FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A35IndexofNearest(Parameter1, Parameter2) Parameter1 is a

ContentsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. Aii3. FunctionsMathematical Functions . . . . . . . . . . . . . . . .

3. FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A36Example: Using NullBetween and NullOutside to Show Selected Po

3.5. Other FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A37In this example, the columns are reporting data directl

3. FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A383.6. NANS/MAKEERRS SoftMax Pro contains a special class of num

3.6. NANs/MakeErrsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A39MakeErr(111) “Path?” Seen in Plate sections when PathChec

3. FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A40The following list contains the functions that can be used to

3.7. Text FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A41If (Values >= MeanValue-(0.2*MeanValue) and Values &l

3. FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A42Example: Combining Text with Numbers to Report a Results Colum

3.7. Text FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A43Keep in mind that after SoftMax Pro converts numbers to

3. FunctionsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A44

Formula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A454. AccessorsAccessors are special functions that provide access to other So

Formula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A11. IntroductionThe Formula Reference Guide for SoftMax Pro software identifi

4. AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A464.1. PLATE/CUVETTESET SETUP INFORMATION ACCESSORSPlate/Cuvette

4.1. Plate/CuvetteSet Setup Information AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A47!ExWavelengths!WellExWavel

4. AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A48!PMTSetting!WellPMTSetting[SpectraMax Gemini, M2, M2e, M5 and

4.2. Kinetic and Spectrum Data AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A49Example: Using Plate Information Ac

4. AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A50!NumPointsRead !WellNumPointsRead [SpectraMax Plus, SpectraMax

4.2. Kinetic and Spectrum Data AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A51!AutomixOn !WellAutomixOnReturns (a

4. AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A52Example: Accessing Kinetic Run Settings Information The !NumPo

4.2. Kinetic and Spectrum Data AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A53We can create a column to calculate

4. AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A54Example: Temperature, Time, and Wavelength Information from Ki

4.3. Plate Data AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A55!WellLmXPRaw (For Fluorescence Polarization readin

1. IntroductionFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A2information). These formulas act on all data from each well

4. AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A56!AllValuesReturns a list of the reduced numbers from all wells

4.3. Plate Data AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A57Example: Dividing All Optical Densities in a Plate

4. AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A58If you have read a CuvetteSet at more than one wavelength, you

4.4. PathCheck AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A59Example: Viewing Pathlength together with Pathlengt

4. AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A60You can use the formulas !PathCheckLm900 and !PathCheckLm1000

4.6. Blank AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A614.6. BLANK ACCESSORSBlank accessors allow you to access

4. AccessorsFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A62Standard deviation of the group blank:Stdev(!GroupBlankValues)

Formula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A63IndexSymbols!A1LmX 54!A1LmXPreRead 56!A1LmXXVals 55!A1Pathlength 58!AllValu

Formula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A64!StdDevPlateBlankLmX 56!StepSweep 50!TemperatureRun 51!TemperatureSetPoint

Formula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A65<> 10( ) 8* 7+ 8, 16/ 7= 9> 9>= 10^ 8~ (tilde) 17AAbs 20Acos 20

1.2. Formula SizeFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A3When writing custom formulas to access raw data in the Plat

Formula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A66IIf 11Index 34IndexListFor 34IndexofMax 34IndexofMin 34IndexofNearest 35In

Formula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A67RRand 22Reduction Formulas 1Referencing Columns 6Referencing Objects in Dif

Formula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A68

1. IntroductionFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A41.3. FORMULA BUILDING BLOCKSSoftMax Pro uses four types of f

1.6. Ordering Functions in Complex FormulasFormula Reference User Guide for SoftMax® Pro Software — 0112-0141 Rev. A5Thus columns in two different Gro